Address:School of Pharmacy, Tokyo University of Pharmacy & Life Science
1432-1 Horinouchi, Hachioji, Tokyo 192-0392 Japan
Fax: (+81)-426-76-4570

CONTENT: [Stafff] [Project] [Publications] [Japanese]

Staff
Name
Professor Fumiyo KUSU, Ph.D.
Associate Professor Hideki HAKAMATA, Ph.D.
Assistant Professor Akira KOTANI, Ph.D.
Assistant Kouji TAKAHASHI, M.S.

Projects

The main theme: Development of Analytical Methods and their Application to Pharmacy and Life Sciences

  1. Analysis of electrode reactions, and their utilization for the development of electrochemical detectors of flow injection analysis (FIA) and high-performance liquid chromatography (HPLC)

  2. Development of high-sensitive methods to determine redox compounds by HPLC with electrochemical detection (HPLC-ECD) using a microbore column

  3. Development of efficient methods to optimize analytical systems by chemometrics
    〜Application of chemometrical methodologies for determining biologically important substances by HPLC-ECD〜

  4. Exploration of signal transduction pathways of cholesterol sensing in cells

  5. Electrochemical identification of marker compounds of oxidative stress in plasma

  6. Development of spectroelectrochemical methods to study electrode reactions

  7. Ion-transfer voltammetry of drugs across immiscible oil/water interfaces

[Top]

Publications

[2007] [2006] [2005] [2004] [2003] [2002] [2001] [2000] [1999] [Reviews]

2007
  1. A. Kotani, K. Takahashi, H. Hakamata, S. Kojima, F. Kusu.
    Attomole catechins determination by capillary liquid chromatography with electrochemical detection.
    Anal. Sci., 23, 157-163 (2007).
  2. A-M. Danila, A. Kotani, H. Hakamata, F. Kusu.
    Determination of rutin, catechin, epicatechin, and epicatechin gallate in buckwheat Fagopyrum esculentum Moench by micro-HPLC with electrochemical detection. J. Agric. Food Chem., 55, 1139-1143 (2007).
  3. K. Hojo, H. Hakamata, A. Ito, A. Kotani, C. Furukawa, Y. Hosokawa, F. Kusu.
    Determination of total cholesterol in serum by high-performance liquid chromatography with electrochemical detection.
    J. Chromatogr. A., 1166, 135-141 (2007).
  4. Y. Aoki, H. Hakamata, Y. Igarashi, K. Uchid, H. Kobayashi, N. Hirayama, A. Kotani, F. Kusu.
    Liquid Chromatography?Tandem Mass Spectrometric Method for Determination of Mosapride Citrate in Equine Tissues.
    J. Chromatogr. B., 858, 135-142 (2007).
2006
  1. K. Takahashi, F. Kusu.
    In Situ Observation of Ferroin and Ferriin in Vicinity of ITO Electrode Surface by Slab Optical Waveguide Spectroscopy.
    Electrochemistry, 74, 192-196 (2006).
  2. A. Kotani, S. Kojima, H. Hakamata, F. Kusu.
    HPLC with Electrochemical Detection to Examine the Pharmacokinetics of Baicalin and Baicalein in Rats after Oral Administration of a Kampo Medicine.
    Anal. Biochem., 350, 99-104 (2006).
  3. A. Kotani, T. Suzuki, A.-M. Danila, H. Shiba, F. Kusu.
    Cyclic Voltammograms of Ferrocyanide, Estrogens, and Rutin at Boron-Doped Diamond and Plastic-Formed Carbon Electrodes.
    ITE Lett., 7, 32-38 (2006).
  4. J. Xia, A. Kotani, H. Hakamata, F. Kusu.
    Determination of Hesperidin in Pericarpium Citri Reticulatae by Semi-Micro HPLC with Electrochemical Detection.
    J. Pharm. Biomed. Anal., 41, 1401-1405 (2006).
  5. 小谷　明，大柴一修，袴田秀樹，高橋浩司，楠　文代．
    日本薬局方における過塩素酸滴定法による定量法の現状と考察 −クロルジアゼポキシドの定量法の滴定法からHPLC-UV法への変更−
    医薬品研究, 37, 497-514 (2006).
2005
  1. A. Kotani, S. Kojima, H. Hakamata, D. Jin, F. Kusu
    Determination of Honokiol and Magnolol by Micro HPLC with Electrochemical Detection and Its Application to the Distribution Analysis in Branches and Leaves of Magnolia obovata.
    Chem. Pharm. Bull., 53, 319-322 (2005).
  2. F. Kusu, K. Oshiba, A. Kotani.
    A Novel Approach to the Sensing of Weak Bases.
    Sensors & Actuators B., 108, 858-862 (2005).
  3. N. Suzuki, N. Miyashita, A. Kotani, F. Kusu, T. Kawasaki.
    Determination of Troglitazone Stereoisomers in Rat Plasma Using Semi-Micro HPLC with Electrochemical Detection.
    J. Pharm. Biomed. Anal., 38, 155-161 (2005).
  4. K. Takahashi, M. Koitabashi, F. Kusu.
    Development of a Novel Electrochemical Cell for Slab Optical Waveguide Spectroscopy for in situ Observation of Methylene Blue and Anions on an Electrode/Electrolyte Interface.
    Talanta, 65, 1120-1125 (2005).
2004
  1. A. Kotani, Y. Miyaguchi, E. Tomita, K. Takamura, F. Kusu.
    Determination of Organic Acids by High-Performance Liquid Chromatography with Electrochemical Detection during Wine Brewing.
    J. Agric. Food Chem., 52, 1440-1444 (2004).
  2. L. Yang, A. Kotani, H. Hakamata, F. Kusu.
    Determination of Ortho-Phenylphenol Residues in Lemon Rind by High-Performance Liquid Chromatography with Electrochemical Detection Using Microbore Column.
    Anal. Sci., 20, 199-203 (2004).
  3. 高橋浩司, 小谷　明, 大槻さなえ，楠　文代
    食品pH調整剤の酸度測定用の電気化学検出フローインジェクション分析
    分析化学，53, 271-274 (2004).
  4. D. Jin, H. Hakamata, K. Takahashi, A. Kotani, F. Kusu.
    Separation of Flavonoids by Semimicro High-Performance Liquid Chromatography with Electrochemical Detection.
    Bull. Chem. Soc. Jpn., 77, 1147-1152 (2004).
  5. M. Nishikawa, N. Ariyoshi, A. Kotani, I. Ishii, H. Nakamura, H. Nakasa, M. Ida, H. Nakamura, N. Kimura, M. Kimura, A. Hasegawa, F. Kusu, S. Ohmori, K. Nakazawa, M. Kitada.
    Effects of Continuous Ingestion of Green Tea or Grape Seed Extracts on the Pharmacokinetics of Midazolam.
    Drug Metab. Pharmacokin., 19, 280-289 (2004).
  6. 北村紀子, 小谷　明, 楠　文代
    ディスポーザブル型電解セルによる木酢液の酸度測定
    分析化学，53, 1097-1000 (2004).
  7. D. Jin, H. Hakamata, K. Takahashi, A. Kotani, F. Kusu.
    Determination of Quercetin in Human Plasma after Ingestion of Commercial Canned Green Tea by Semi-Micro High-Performance Liquid Chromatography with Electrochemical Detection.
    Biomed. Chromatogr., 18, 662-666 (2004).
  8. F. Kusu, K. Oshiba, A. Kotani.
    A Novel Approach to the Sensing of Weak Bases.
    Chem. Sens., 20B, 122-123 (2004).
2003
  1. Akira Kotani, Yuzuru Hayashi, Rieko Matsuda and Fumiyo Kusu.
    Prediction of Measurement Precision of Apparatus Using a Chemometric Tool in Electrochemical Detection of High-Performance Liquid Chromatography.
    J. Chromatogr. A., 986, 239 (2003).
  2. Akira Kotani, Naoto Miyashita and Fumiyo Kusu.
    Determination of Catechins in Human Plasma after Commercial Canned Green Tea Ingestion by High-Performance Liquid Chromatography with Electrochemical Detection Using a Microbore Column.
    J. Chromatogr. B., 788, 269 (2003).
  3. Akira Kotani, Yuzuru Hayashi, Rieko Matsuda and Fumiyo Kusu.
    Optimization of HPLC-ECD Conditions for Determination of Catechins with Precision and Efficiency Based on the FUMI Theory.
    Anal. Sci., 19, 865 (2003).
  4. 塚本　龍治，楠　文代
    ヒスタミンと抗ヒスタミン薬の油水界面における イオン移動ボルタンメトリー
    分析化学, 52, 865 (2003).
  5. Junichi Taguchi, Sanae Ohtsuki, and Fumiyo Kusu.
    Voltammetric Determination of Weak Bases Based on Oxidation of α-tocopherol in an Unbuffered Solution.
    J. Electroanal. Chem., 557, 91-97 (2003).
  6. Akira Kotani, Yuji Miyaguchi, Dai Harada, and Fumiyo Kusu.
    A Disposable Voltammetric Cell for Determining the Titratable Acidity in Vinegar.
    Anal. Sci., 19, 1473-1476 (2003).
  7. Akira Kotani, Takeshi Iwagami, Taisuke Ueda, Yoshio Kimura, Yuzuru Hayashi, Rieko Matsuda, and Fumiyo Kusu.
    Prediction of Precision Based on FUMI Theory and a Single Test - Effect of Thickness of Radial Flow Cell on Electrochemical Detection using Micro HPLC ?
    ITE Lett., 4, 783-787 (2003).
  8. 小板橋基子, 小谷　明, 楠　文代
    電気化学検出フローインジェクション分析法によるリドカインの定量
    分析化学, 52, 1137-1140 (2003).
2002
  1. Akira Kotani and Fumiyo Kusu.
    HPLC with Electrochemical Detection for Determining the Distribution of Free Fatty Acids in Skin Surface Lipids from the Human Face and Scalp.
    Arch. Dermatol. Res., 294, 172 (2002).
  2. 篠原　徹，小谷　明，楠　文代
    電気化学発光検出/フローインジェクション分析法によるグルコース及び尿酸の高感度定量
    分析化学，51, 423 (2002).
  3. 宮口裕二, 小谷　明, 楠　文代
    電気化学検出高速液体クロマトグラフィーによる血中乳酸の定量
    分析化学, 51, 703-706 (2002).
  4. Fielding PE, Russel JS, Spencer TA, Hakamata H, Nagao K, Fielding CJ.
    Sterol efflux to apolipoprotein A-I originates from caveolin-rich microdomains and potentiates PDGF-dependent protein kinase activity.
    Biochemistry. 41, 4929 (2002).
  5. Ohgami N, Nagai R, Ikemoto M, Arai H, Miyazaki A, Hakamata H, Horiuchi S, Nakayama H.
    CD36, serves as a receptor for advanced glycation endproducts (AGE).
    J Diabetes Complications. 16, 56 (2002).

2001

  1. Bin Yang, Akira Kotani, Kensuke Arai and Fumiyo Kusu.
    Estimation of the Antioxidant Activities of Flavonoids from Their Oxidation Potentials.
    Anal. Sci., 17, 599 (2001).
  2. Bin Yang, Kensuke Arai and Fumiyo Kusu.
    Electrochemical Behaviors of Quercetin and Kaempferol in Neutral Buffer Solution.
    Anal. Sci., 17, 987 (2001).
  3. Bin Yang, Kensuke Arai and Fumiyo Kusu.
    Oxidation Potentials of Flavonoids Determined by Flow-through Column Electrolysis.
    Electrochem., 69, 519 (2001).
  4. Bin Yang, Akira Kotani, Kensuke Arai and Fumiyo Kusu.
    Relationship of Electrochemical Oxidation of Catechins on Their Antioxidant Activity in Microsomal Lipid Peroxidation.
    Chem. Pharm. Bull., 49, 747 (2001).
  5. Sanae Ohtsuki, Naoto Kunimatsu, Kiyoko Takamura and Fumiyo Kusu.
    Determination of the Total Acid Content in Wine Based on the Voltammetric Reduction of Quinone.
    Electroanalysis, 13, 404 (2001).

2000

  1. Kensuke Arai, Takayuki Sato, Hiromitsu Tamanouchi, Maria Ohtomo, Kiyoko Takamura, Tetsuro Sueoka and Fumiyo Kusu.
    Electrolysis of 2-Methyl-1,4-naphthoquinone and 3,5-Di-tert-butyl-1,2-benzoquinone Using the Flow-Cell System with a Column Electrode.
    Electrochim. Acta, 45, 3029 (2000).
  2. Tetsuo Fuse, Go ODA, Kensuke Arai, Kiyoko Takamura and Fumiyo Kusu.
    Electrochemical Determination of Lipase Activity in Pharmaceutical Praparations.
    Electrochim. Acta, 45, 2933 (2000).
  3. Akira Kotani Tetsuo Fuse, and Fumiyo Kusu.
    Determination of Plasma Free Fatty Acids by High-Performance Liquid Chromatography with Electrochemical Detection.
    Anal. Biochem., 284, 65 (2000).
  4. Bin Yang, Kensuke Arai and Fumiyo Kusu.
    Determination of Catechins in Human Urine Subsequent to Tea Ingestion by High-Performance Liquid Chromatography with Electrochemical Detection.
    Anal. Biochem., 283, 77 (2000).
  5. Keiji Ohe and Fumiyo Kusu.
    Metal Ion Elution from Dental Alloys Due to Compound Iodine Glycerin in Artificial Saliva.
    Electrochem., 68, 582 (2000).
  6. Keiji Ohe and Fumiyo Kusu.
    Surface of Precious Dental Alloys in Artificial Saliva Observed by In Situ Specular Reflection Spectroscopy.
    Bunseki Kagaku, 49, 503 (2000).
  7. Fielding PE, Nagao K, Hakamata H, Chimini G, Fielding CJ.
    A two-step mechanism for free cholesterol and phospholipid efflux from human vascular cells to apolipoprotein A-1.
    Biochemistry, 39, 14113 (2000).
  8. Ohgami N, Kuniyasu A, Furukawa K, Miyazaki A, Hakamata H, Horiuchi S, Nakayama H. Glibenclamide acts as an inhibitor of acyl-CoA:cholesterol acyltransferase enzyme.
    Biochem. Biophys. Res. Commun., 277, 417 (2000).
  9. Pullinger CR, Hakamata H, Duchateau PN, Eng C, Aouizerat BE, Cho MH, Fielding CJ, Kane JP.
    Analysis of hABC1 gene 5' end: additional peptide sequence, promoter region, and four polymorphisms.
    Biochem. Biophys. Res. Commun., 271, 451 (2000).
  10. Biwa T, Sakai M, Matsumura T, Kobori S, Kaneko K, Miyazaki A, Hakamata H, Horiuchi S, Shichiri M. Sites of action of protein kinase C and phosphatidylinositol 3-kinase are distinct in oxidized low density lipoprotein-induced macrophage proliferation.
    J. Biol. Chem., 275, 5810 (2000).

1999

  1. Kensuke Arai, Kouji Takahashi and Fumiyo Kusu.
    An Electrochemiluminescence Flow-Through Cell and Its Application to Sensitive Immunoassay Using N-(Aminobutyl)-N-ethylisoluminol.
    Anal. Chem., 71, 2237 (1999).
  2. Kensuke Arai, Kakujirou Watanabe and Fumiyo Kusu.
    Effects of Bitter Antibiotics on Electrical Potential Oscillation across a Water/Octanol/Water Liquid Membrane.
    Trans. IEE Japan, 119-E, 544 (1999).
  3. Han H, Sasaki J, Matsunaga A, Hakamata H, Huang W, Ageta M, Taguchi T, Koga T, Kugi M, Horiuchi S, Arakawa K.
    A novel mutant, ApoA-I nichinan (Glu235-->0), is associated with low HDL cholesterol levels and decreased cholesterol efflux from cells.
    Arterioscler. Thromb. Vasc. Biol., 19, 1447 (1999).

Reviews

  1. Akira Kotani, Fumiyo Kusu, and Kiyoko Takamura.
    New Electrochemical Detection Method in High-Performance Liquid Chromatography for Determining Free Fatty acids.
    Anal. Chim. Acta., 465, 199 (2002).
  2. Kensuke Arai and Fumiyo Kusu.
    Electrical Potential Oscillation across a Water-Oil-Water Liquid Membrane in the Presence of Drugs.
    in Liquid Interfaces in Chemical, Biological, and Pharmaceutical Applications (Edited by A. G. Volkov), pp. 699-727, Marcel Dekker, New York (2001).
  3. Kiyoko Takamura, Tetsuo Fuse, Kensuke Arai and Fumiyo Kusu.
    A Review of a New Voltammetric Method for Determining Acids.
    J. Electroanal. Chem., 468, 53 (1999).
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